Cell Fractionation Golgi bottom fraction

Creator:

Bergeron, John
Palade, George E
Siekevitz, Philip

Published/Created:

New Haven, CT
Yale University School of Medicine
11/11/1970

Physical Description:

3.25 in. x 4 in.

Related Links:

http://www.jcb.org/cgi/content/abstract/59/1/45

Notes:

3.25 in. x 4 in. Lantern Slides
Original Magnification: x13,750
The authors took advantage of the fact that rats gavage-fed a large bolus of ethanol (single malt I hope) were observed to have their Golgi cisternae loaded with LDL particles. Since LDL particles are lighter than most other intracellular organelles, following homogenization of ethanol-fed rats, the Golgi fractions "floated" to the top of denser sucrose gradients during centrifugation. This allowed investigators to analyze the membrane properties of "pure" Golgi fractions and set the stage for development of fusion assays that led to an understanding of factors regulating the specificity of membrane fusion - the SNARE hypothesis.

Topics:

Cell Fractionation

Topics:

Jamieson, James D

Genre:

transmission electron micrograph

Format:

Image

Content Type:

Prints & Photographs

Rights:

The use of this image may be subject to the copyright law of the United States (Title 17, United States Code) or to site license or other rights management terms and conditions. The person using the image is liable for any infringement
Yale Community Only

Access Restrictions:

Public

Yale Collection:

Cushing/Whitney Medical Library

Digital Collection:

George E. Palade Electron Microscopy Slide Collection

Citation:

See Ehrenreich, J.H., J.J.M. Bergeron, P. Siekevitz and G.E. Palade. 1973. Golgi fractions prepared from rat liver homogenates: I. Isolation procedure and morphological characterization. J. Cell. Biol. 59:45-72.

OID:

15817825

PID:

digcoll:2795256